Formate Dehydrogenase P1
 from Pseudomonas spec. (recombinant in E.Coli)
 EC 1.2.1.2
 [9028-85-7]

Characteristics
Formate:NAD oxidoreductase
5 U/mg
very stable at 4 ^oC (in 1 M ammonium sulfate and 10% glycerol)

Substrate Spectrum
Specific for formate (K_m = 10 mM) and NAD (K_m = 0.08 mM)
very low activity with NADP (K_m = 100 mM)

Application
Cofactor regeneration of NADH in reductive processes
The enzyme is active within a range of 6.0 < pH < 9.3

Quantities
10 kU, 100 kU, 1000 kU